Abstract
Cultured epithelial cells obtained from guinea pig tracheal preparations metabolized arachidonic acid into 5- and 15-hydroxy-eicosatetraenoic acid and into the prostaglandins E2 and F2 alpha. Linoleic acid was converted by the epithelial cells into 9-hydroxy-octadecadienoic acid (9-HODE) and smaller amounts of 13-HODE. It was further investigated whether linoleic acid metabolites are of importance for the regulation of airway smooth muscle function. 13-HODE caused an increase of maximal contraction of tracheal rings to histamine, while 9-HODE had no effect.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Epithelial Cells
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Epithelium / metabolism
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Epithelium / physiology
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Histamine / pharmacology
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Linoleic Acid
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Linoleic Acids / metabolism*
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Linoleic Acids / pharmacology
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Linoleic Acids, Conjugated*
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Muscle Contraction / drug effects
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Muscle, Smooth / cytology
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Muscle, Smooth / metabolism
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Muscle, Smooth / physiology*
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Trachea / cytology
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Trachea / metabolism
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Trachea / physiology*
Substances
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Linoleic Acids
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Linoleic Acids, Conjugated
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9-hydroxy-10,12-octadecadienoic acid
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13-hydroxy-9,11-octadecadienoic acid
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Histamine
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Linoleic Acid