Abstract
The biochemical and molecular mechanisms used by alkaliphilic bacteria to acquire iron are unknown. We demonstrate that alkaliphilic (pH > 9) Bacillus species are sensitive to artificial iron (Fe³+) chelators and produce iron-chelating molecules. These alkaliphilic siderophores contain catechol and hydroxamate moieties, and their synthesis is stimulated by manganese(II) salts and suppressed by FeCl₃ addition. Purification and mass spectrometric characterization of the siderophore produced by Caldalkalibacillus thermarum failed to identify any matches to previously observed fragmentation spectra of known siderophores, suggesting a novel structure.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacillaceae / metabolism*
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Catechols / analysis
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Chlorides / metabolism
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Ferric Compounds / metabolism
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Hydroxamic Acids / analysis
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Iron / metabolism*
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Iron Chelating Agents / chemistry
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Iron Chelating Agents / isolation & purification
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Iron Chelating Agents / metabolism
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Manganese / metabolism
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Mass Spectrometry
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Siderophores / chemistry
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Siderophores / isolation & purification
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Siderophores / metabolism
Substances
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Catechols
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Chlorides
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Ferric Compounds
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Hydroxamic Acids
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Iron Chelating Agents
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Siderophores
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Manganese
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Iron
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catechol
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ferric chloride