A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential

Daniel R Gentry; Imogen Wilding; John M Johnson; Dongzhao Chen; Katja Remlinger; Cindy Richards; Susan Neill; Magdalena Zalacain; Stephen F Rittenhouse; Michael N Gwynn

doi:10.1016/j.mimet.2010.08.012

A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential

J Microbiol Methods. 2010 Nov;83(2):254-6. doi: 10.1016/j.mimet.2010.08.012. Epub 2010 Aug 27.

Authors

Daniel R Gentry¹, Imogen Wilding, John M Johnson, Dongzhao Chen, Katja Remlinger, Cindy Richards, Susan Neill, Magdalena Zalacain, Stephen F Rittenhouse, Michael N Gwynn

Affiliation

¹ Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA 19426, United States. dan.r.gentry@gsk.com

PMID: 20801170
DOI: 10.1016/j.mimet.2010.08.012

Abstract

We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.

Publication types

Validation Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteriological Techniques / methods*
Carbocyanines / metabolism
Escherichia coli / drug effects
High-Throughput Screening Assays / methods*
Inhibitory Concentration 50
Membrane Potentials / drug effects*
Staphylococcus aureus / drug effects*

Substances

Anti-Bacterial Agents
Carbocyanines
3,3'-diethyloxacarbocyanine