A rapid enzyme immunoassay system was developed for the detection and quantitative measurement of Total IgE and specific IgE in human serum. The system utilizes a unique solid phase configuration called Acti Tip for the separation phase and an enzyme base detection system. Measurement and quantitation of Total IgE and allergen specific IgE is achieved through the use of a compact photometer pregrogrammed to print out IgE results in units and classes. Only 10 microliters of serum and 45 minutes reaction time is required to measure Total IgE. Allergen specific IgE antibodies are detected and quantitated in 4 hours. A very good agreement exists between test results obtained by the Acti Tip Allerg E and Allerg Ens test systems and the Pharmacia Phadebas PRIST and RAST tests. The correlation coefficients between the two systems is in excess of 90%. The Dexall Acti Tip system offers a number of significant advantages over the currently available in vitro techniques for the detection and quantitation of Total IgE and allergen specific IgE.