In 254 findings of the acid-base balance ions and proteins, the authors evaluated mutual relations between the acid-base balance and calculation from the anion column. Contrary to the commonly used anion gap value, the authors used concurrently for evaluation buffer base sera, serum protein values and residual anions. The logical result of summarization of these three parameters is the creation of a new term, delta bases, which correlates closely with the resulting metabolic acid-base situation. The optimal relationship of the mentioned parameters to the value of base excess is described by the following equation: BE = 0.805*BBS--0.852*RA--0.204*SProt--15.8 which is consistent with the theoretical value of delta bases.