Label-free detection of DNA hybridization using surface enhanced Raman spectroscopy

J Am Chem Soc. 2010 Sep 22;132(37):12792-3. doi: 10.1021/ja105678z.

Abstract

The SERS spectrum of DNA is strongly dominated by the strong spectral feature of adenine at 736 cm(-1); the presence of adenine can serve as an endogenous marker for the label-free SERS-based detection of DNA hybridization when the probe DNA sequence is adenine-free. The substitution of 2-aminopurine for adenine on the probe DNA sequence enables the detection of a target sequence using SERS, upon hybridization of the target with the 2-AP-substituted probe DNA sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / analysis*
  • DNA / chemistry*
  • DNA / genetics
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / analysis
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / genetics
  • Spectrum Analysis, Raman*
  • Surface Properties

Substances

  • Oligodeoxyribonucleotides
  • DNA