Abstract
To get insight into molecular mechanisms governing plant development, the dynamics of abundance and cellular localisation of signalling components need to be understood. Luciferase and green fluorescent protein (GFP)-derived reporters are suitable markers to determine dynamic signalling processes in vivo. Here, analysis of phytochrome A (phyA) photoreceptor dynamics during early seedling development is used as an example of how in vitro and in vivo luciferase assays as well as GFP-imaging can be used to probe signalling dynamics.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Gene Expression Regulation, Plant*
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Genes, Reporter*
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Green Fluorescent Proteins / genetics*
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Luciferases / genetics*
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Microscopy, Fluorescence / methods
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Photoreceptors, Plant / genetics*
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Photoreceptors, Plant / metabolism
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Phytochrome A / genetics*
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Phytochrome A / metabolism
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Plant Development
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plants / genetics*
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Plants / metabolism
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Seedlings / genetics
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Seedlings / growth & development
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Seedlings / metabolism
Substances
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Photoreceptors, Plant
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Phytochrome A
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Plant Proteins
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Green Fluorescent Proteins
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Luciferases