Differences in sensitivity to HMME-mediated photodynamic therapy between EBV+ C666-1 and EBV- CNE2 cells

Photodiagnosis Photodyn Ther. 2010 Sep;7(3):204-9. doi: 10.1016/j.pdpdt.2010.05.001. Epub 2010 Jun 22.

Abstract

Objectives: The aim of this study was to compare the sensitivity of hematoporphyrin monomethyl ether (HMME)-mediated PDT in the Epstein-Barr virus (EBV) positive and negative human nasopharyngeal carcinoma (NPC) cells.

Methods and materials: The intracellular uptake of HMME in C666-1 (EBV+) and CNE2 (EBV-) cells was quantitatively determined after incubating with different concentration of HMME. The subcellular localization of HMME was examined under a laser scanning fluorescence microscopy. The survival rate of C666-1 and CNE2 cells after HMME PDT was evaluated with clonogenic assay.

Results: The EBV- CNE2 cells showed higher intracellular uptake of HMME and lower survival rate than EBV+ C666-1 cells. There was no significant difference in terms of subcellular localization of HMME between C666-1 and CNE2 cells.

Conclusion: The sensitivity of EBV- CNE2 cells to HMME-mediated PDT was significantly higher than that of EBV+ C666-1 cells. The intracellular uptake of HMME plays a key role in determining the PDT sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Hematoporphyrins / pharmacokinetics
  • Hematoporphyrins / pharmacology*
  • Herpesvirus 4, Human*
  • Humans
  • Nasopharyngeal Neoplasms / metabolism*
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / virology
  • Photochemotherapy
  • Photosensitizing Agents / pharmacokinetics
  • Photosensitizing Agents / pharmacology*

Substances

  • Hematoporphyrins
  • Photosensitizing Agents
  • hematoporphyrin monomethyl ether