NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role

FEBS Lett. 2010 Sep 24;584(18):3916-22. doi: 10.1016/j.febslet.2010.08.019. Epub 2010 Aug 18.

Abstract

NfrA1 nitroreductase from the Gram-positive bacterium Bacillus subtilis is a member of the NAD(P)H/FMN oxidoreductase family. Here, we investigated the reactivity, the structure and kinetics of NfrA1, which could provide insight into the unclear biological role of this enzyme. We could show that NfrA1 possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide. Finally, we showed that NfrA1 is able to rapidly scavenge H(2)O(2) produced during the oxidative process or added exogenously.

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / enzymology*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology*
  • Cloning, Molecular
  • Hydrogen Peroxide / metabolism*
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics
  • Multienzyme Complexes / physiology*
  • NAD / metabolism
  • NADH, NADPH Oxidoreductases / chemistry
  • NADH, NADPH Oxidoreductases / genetics
  • NADH, NADPH Oxidoreductases / physiology*
  • Niacinamide / biosynthesis
  • Nitroreductases / chemistry
  • Nitroreductases / genetics
  • Nitroreductases / physiology*
  • Oxidative Stress
  • Protein Conformation
  • Superoxides / metabolism

Substances

  • Bacterial Proteins
  • Multienzyme Complexes
  • NAD
  • Superoxides
  • Niacinamide
  • Hydrogen Peroxide
  • NADH oxidase
  • NADH, NADPH Oxidoreductases
  • Nitroreductases
  • NfrA1 protein, Bacillus subtilis

Associated data

  • PDB/3N2S