Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography

J Lipid Res. 1991 May;32(5):877-9.

Abstract

Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Centrifugation
  • Chromatography, Thin Layer / methods*
  • Fatty Acids / analysis
  • Gas Chromatography-Mass Spectrometry
  • Hydrolysis
  • Lipids / chemistry
  • Lipids / isolation & purification*
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Phospholipids / chemistry
  • Phospholipids / isolation & purification*

Substances

  • Fatty Acids
  • Lipids
  • Phospholipids