Single-copy flanking sequences in human histone gene clusters map to chromosomes 1 and 6

B S Allen; J L Stein; G S Stein; H Ostrer

doi:10.1016/0888-7543(91)90337-e

Single-copy flanking sequences in human histone gene clusters map to chromosomes 1 and 6

Genomics. 1991 Jun;10(2):486-8. doi: 10.1016/0888-7543(91)90337-e.

Authors

B S Allen¹, J L Stein, G S Stein, H Ostrer

Affiliation

¹ Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610.

PMID: 2071153
DOI: 10.1016/0888-7543(91)90337-e

Abstract

Two single-copy sequences flanking two different human histone gene clusters were used as probes to map these clusters by in situ hybridization. pFF435B, a unique sequence subclone derived from a lambda genomic clone (lambda HHG55) containing H2A, H2B, H3, and H4 genes, mapped to chromosome 1q21 (chi 2 = 120.99, P less than 0.001). pST519E, a single-copy sequence derived from a lambda genomic clone (lambda HHG17) containing only H3 and H4 genes, mapped to chromosome 6p21 (chi 2 = 112.62, P less than 0.001). These findings agree with previous assignments of human histone genes to chromosomes 1 and 6 and demonstrate that the single-copy flanking sequences in different human histone gene clusters are unique for different chromosomes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Human, Pair 1*
Chromosomes, Human, Pair 6*
Histones / genetics*
Humans
Introns
Multigene Family*
Nucleic Acid Hybridization
Restriction Mapping

Substances

Histones