Based on 3S technique, this paper examined the responses of ecosystem service values (ESVs) to the landscape pattern change in the typical Karst area of northwest Guangxi, China in 1985, 1990, 2000 and 2005. The ESVs in the study area had close relations to landscape area, fragmentation degree, complexity of patch shape, areas of critical type, patch connectivity, and patch richness. It was linearly positively correlated with landscape area and had curvilinear positive correlations with patch index (LPI), contagion index (CONTAG), aggregative index (AI), effective mesh (MESH), proportion of like adjacencies (PLADJ), and tended to be increased with increasing patch area and patch connectivity of critical landscape type. The ESVs had curvilinear negative correlations with division index (DIVISION), split index (SPLIT), and patch richness (PRD), and decreased with increasing patch fragmentation and shrinking patch size. Therefore, it would be important to protect the critical landscape types such as woodland, shrub, and grassland, and to increase the patch size and connectivity to 'avoid further fragmentation. Moreover, it would be necessary to reduce the frequency and severity of disturbances to ensure the ESVs growth and the sustainable development of the study area.