Anthelmintic activity of Pistacia lentiscus foliage in two Middle Eastern breeds of goats differing in their propensity to consume tannin-rich browse

Abstract

The Damascus and Mamber breeds of goats thrive in Middle Eastern Mediterranean regions where the tannin-rich (20% of polyethylene glycol-binding tannins) brush species Pistacia lentiscus L. (lentisk) is ubiquitous. In light of the increasing recognition of the anthelmintic activity of plant tannins, we examined the effect of offering lentisk foliage for 24 days on fecal egg excretion in 5.5-month-old Damascus and Mamber kid goats (n=28) following infection with 10,000 L3 larvae of mixed gastro-intestinal nematodes (GIN). Lentisk foliage was consumed with or without a daily supplement of 20 g polyethylene glycol (PEG; MW 4000). Lentisk tannins showed a strong protein-depletive effect that was totally reversed by the addition of PEG. At the peak of infection, kids of the two breeds lost weight unless they were fed with lentisk without PEG. Fecal egg counts (FEC) were lowest - and did not differ from 0 - in kids fed lentisk without PEG, highest in the controls fed hay as roughage, and intermediate in kids fed lentisk and PEG (241, 1293, and 705 eggs per gram, respectively, SEM 180; P<0.001); therefore, the anthelmintic activity of lentisk was only partly attributable to tannins. The suppressive effect of lentisk on FEC ceased when feeding was discontinued, suggesting that female parasites were not killed but their fertility was reversibly impaired. Damascus kids showed lower FEC than their Mamber counterparts, inferring that the effect of foraging on tannin-rich species is only additive to genetic differences between goat breeds in their sensitivity to GIN infection. On the basis of our results we would expect yearlong lentisk grazing to result in no or very low GIN infection, and Damascus goats to have some advantage over Mamber goats where chemical control of GIN is unfeasible. There appears to be a trade-off between the benefits of lentisk tannin as drug and its side-effects (protein depletion) when given at high level; how goats balance this trade-off requires further elucidation.