Pulse wave velocity (PWV) is a useful method to assess arterial stiffness and predict mortality of atherosclerosis-related diseases. The progression of atherosclerosis is not homogeneous. There must be difference of PWV between sites by site. Therefore we designed a multi-channel instrument to measure PWV at different sites of the body simultaneously. We measured PWV at six different regions simultaneously. Thirty four healthy adults received the measurement. We found that PWVs were higher in the large vessels, by measuring from the heart to toes and heart to fingers as compared with the measurement from the heart to the earlobes (4.76 ± 0.46 m/s; 4.67 ± 0.41 m/s; 1.10 ± 0.16 m/s). The PWV of the left and right sides were the same. Although there were statistically significances, the correlation of PWV between foot and hand is better than those between ear and foot and between ear and hand. Herein we presented a novel and reliable measurement of PWV. The changes of PWV in different regions may be used in predicting disease processes such as stroke, coronary artery diseases and renal diseases, respectively.