Actin-based motility of Burkholderia thailandensis requires a central acidic domain of BimA that recruits and activates the cellular Arp2/3 complex

Chayada Sitthidet; Joanne M Stevens; Terence R Field; Abigail N Layton; Sunee Korbsrisate; Mark P Stevens

doi:10.1128/JB.00608-10

Actin-based motility of Burkholderia thailandensis requires a central acidic domain of BimA that recruits and activates the cellular Arp2/3 complex

J Bacteriol. 2010 Oct;192(19):5249-52. doi: 10.1128/JB.00608-10. Epub 2010 Aug 6.

Authors

Chayada Sitthidet¹, Joanne M Stevens, Terence R Field, Abigail N Layton, Sunee Korbsrisate, Mark P Stevens

Affiliation

¹ Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkoknoi, Bangkok 10700, Thailand.

Abstract

Burkholderia species use BimA for intracellular actin-based motility. Uniquely, Burkholderia thailandensis BimA harbors a central and acidic (CA) domain. The CA domain was required for actin-based motility, binding to the cellular Arp2/3 complex, and Arp2/3-dependent polymerization of actin monomers. Our data reveal distinct strategies for actin-based motility among Burkholderia species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Burkholderia / genetics
Burkholderia / metabolism*
Electrophoresis, Polyacrylamide Gel
Microfilament Proteins / genetics
Microfilament Proteins / metabolism*
Microscopy, Confocal

Substances

Actins
Bacterial Proteins
Microfilament Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding