Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor

Ken'ichiro Matsumoto; Kei Kitagawa; Sung-Jin Jo; Yuyang Song; Seiichi Taguchi

doi:10.1016/j.jbiotec.2010.07.031

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor

J Biotechnol. 2011 Apr 10;152(4):144-6. doi: 10.1016/j.jbiotec.2010.07.031. Epub 2010 Aug 6.

Authors

Ken'ichiro Matsumoto¹, Kei Kitagawa, Sung-Jin Jo, Yuyang Song, Seiichi Taguchi

Affiliation

¹ Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan. mken@eng.hokudai.ac.jp

PMID: 20692303
DOI: 10.1016/j.jbiotec.2010.07.031

Abstract

Lipopolysaccharides free P[3-hydroxybutyrate (3HB)-co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8-47wt% of P(3HB-co-3HV). The 3HV fraction in the copolymer was varied from 0 to 28mol% depending on the propionate concentrations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors*
Biotechnology / methods*
Cell Culture Techniques
Corynebacterium glutamicum / metabolism*
Cupriavidus necator / genetics
Polyesters / metabolism*
Polyhydroxyalkanoates / biosynthesis
Polyhydroxyalkanoates / genetics*
Propionates / metabolism

Substances

Polyesters
Polyhydroxyalkanoates
Propionates
poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)