Systems involved in K+ uptake from diluted solutions in pepper plants as revealed by the use of specific inhibitors

Abstract

Here, the contribution of the HAK1 transporter, the AKT1 channel and a putative AtCHX13 homolog to K(+) uptake in the high-affinity range of concentrations in pepper plants was examined. The limited development of molecular tools in pepper plants precluded a reverse genetics study in this species. By contrast, in the model plant Arabidopsis thaliana, these type of studies have shown that NH(4)(+) and Ba(2+) may be used as specific inhibitors of the two K(+) uptake systems to dissect their contribution in species in which, as in pepper, specific mutant lines are not available. By using these inhibitors together with Na(+) and Cs(+), the relative contributions of CaHAK1, CaAKT1 and a putative AtCHX13 homolog to K(+) acquisition from diluted solutions under different regimens of K(+) supply were studied. The results showed that, in plants completely starved of K(+), the gene encoding CaHAK1 was highly expressed and this system is a major contributor to K(+) uptake. However, K(+) concentrations as low as 50μM reduced CaHAK1 expression and the CaAKT1 channel came into play, participating together with CaHAK1 in K(+) absorption. The contribution of a putative AtCHX13 homolog seemed to be low under this low K(+) supply, but it cannot be ruled out that at higher K(+) concentrations this system participates in K(+) uptake. Studies of this type allow extension of the tools developed in model plants to understand nutrition in important crops.