Removal of Salmonella Enteritidis from commercial unpasteurized liquid egg white using pilot scale cross flow tangential microfiltration

Int J Food Microbiol. 2010 Sep 1;142(3):309-17. doi: 10.1016/j.ijfoodmicro.2010.07.009. Epub 2010 Jul 15.

Abstract

Effectiveness of a cross flow microfiltration (MF) process for removal of a cocktail of Salmonella enterica serovar Enteritidis species from commercial unpasteurized liquid egg white (LEW) from a local egg breaking plant, while maintaining its functional properties was evaluated. To facilitate MF, LEW was wedge screened, homogenized and then diluted (1:2 w/w) with distilled water containing 0.5% sodium chloride. Diluted unpasteurized LEW was inoculated with five strains of S. Enteritidis (ATCC 4931, ATCC BAA-708, ATCC 49215, ATCC 49218, and ATCC BAA-1045) to a level of approximately 10(7)CFU/mL of LEW and microfiltered using a ceramic membrane. Process parameters influencing egg white functional properties and pathogen removal efficiency were evaluated. Average permeates flux increased by almost 126% when pH of LEW was adjusted from pH 8 to pH 7 at 25 degrees C. Microbial removal efficiency was at least, on average, 6.8Log(10)CFU/mL (limit of detection < or =0.5Log(10)CFU/mL). Functional property analysis indicated that the MF process did not alter the foaming power of LEW.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ceramics
  • Colony Count, Microbial
  • Consumer Product Safety
  • Egg White / microbiology*
  • Filtration / methods*
  • Food Contamination / prevention & control
  • Food Handling / methods
  • Food-Processing Industry / methods*
  • Humans
  • Membranes, Artificial
  • Pilot Projects
  • Salmonella Food Poisoning / prevention & control*
  • Salmonella enteritidis / isolation & purification*

Substances

  • Membranes, Artificial