Contribution of hydrogen peroxide to the inhibition of Staphylococcus aureus by Lactococcus garvieae in interaction with raw milk microbial community

Abstract

The response of Staphylococcus aureus growth inhibition by Lactococcus garvieae to catalase and milk lactoperoxidase, and its efficiency in raw milk cheese were evaluated. S. aureus and L. garvieae were co-cultivated in broth buffered at pH 6.8, and in raw, pasteurized and microfiltered milk, in presence and absence of catalase. Although H2O2 production by L. garvieae was detected only in agitated broth, the inhibition of S. aureus by L. garvieae was reduced by catalase both in static and shaking cultures by 2.7 log, pasteurized milk (approximately 0.7 log), microfiltered milk (approximately 0.6 log) and raw milk (approximately 0.2 log). The growth of S. aureus alone in microfiltered milk was delayed compared with that in pasteurized milk and inhibition of S. aureus by L. garvieae was stronger in microfiltered milk. The inhibition of coagulase-positive staphylococci (CPS) by L. garvieae in raw milk cheese was similar to that in raw milk (approximately 0.8 log), but weaker than that in pasteurized and microfiltered milks. L. garvieae also had an early antagonistic effect on the growth of several other microbial groups, which lastingly affected populations levels and balance during cheese ripening.