Characterization of emtricitabine related substances by liquid chromatography coupled to an ion trap mass spectrometer

Murali Pendela; Dunge Ashenafi Mamade; Jos Hoogmartens; Ann Van Schepdael; Erwin Adams

doi:10.1016/j.talanta.2010.04.004

Characterization of emtricitabine related substances by liquid chromatography coupled to an ion trap mass spectrometer

Talanta. 2010 Jun 30;82(1):125-8. doi: 10.1016/j.talanta.2010.04.004. Epub 2010 Apr 13.

Authors

Murali Pendela¹, Dunge Ashenafi Mamade, Jos Hoogmartens, Ann Van Schepdael, Erwin Adams

Affiliation

¹ Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, Laboratorium voor Farmaceutische Analyse, O&N2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.

PMID: 20685446
DOI: 10.1016/j.talanta.2010.04.004

Abstract

Emtricitabine (FTC) is an antiretroviral compound that inhibits the HIV-1 reverse transcriptase. For the quantification of FTC related substances, a liquid chromatography (LC) method coupled with ultraviolet (UV) detection was developed earlier in our laboratory. Several unknown compounds were detected during the analysis of a commercial sample. However, most of these impurities were not characterized. In this study, impurity profiling in a selected FTC sample was done using LC-mass spectrometry (MS). Due to the presence of a non-volatile buffer, a desalting procedure was carried out before sending the impurity into the MS. Totally, nine peaks were studied and most of them could be characterized.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods*
Deoxycytidine / analogs & derivatives*
Deoxycytidine / chemistry
Emtricitabine
Mass Spectrometry / methods*
Reverse Transcriptase Inhibitors / chemistry*
Salts / chemistry
Salts / isolation & purification

Substances

Reverse Transcriptase Inhibitors
Salts
Deoxycytidine
Emtricitabine