Background and objective: Transient receptor potential canonical (TRPC) proteins, a group of Ca2' permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC).
Methods: Real-time quantitative PCRwas performed to screen the expression of TRPC mRNA in NSCLC tissue. Protein expression of TRPC was detected by Western blot.
Results: Among the seven family members of TRPC so far identified (TRPC1-7), we detected the expression ofTRPC1, TRPC3, TRPC4, TRPC6 mRNA in 24 cases of NSCLC tissue; TRPC2, TRPC5 and TRPC7 mRNA were not detectable. The relative abundance of the expressed TRPC was TRPC1 approximately equal TRPC6 > TRPC3 > TRPC4. Western blot confirmed the protein expression of TRPC1, TRPC3, TRPC4 and TRPC6 in NSCLC tissue.
Conclusion: Out of the seven members of TRPC, we found TRPC1, TRPC3, TRPC4, TRPC6 mRNA and protein were selectively expressed in human NSCLC tissue. This study could provide a basis for future exploration of the individual role of these TRPC proteins in mediating SOCE and in the progression of lung cancer.
背景与目的: 经典瞬时受体电位（transient receptor potential canonical, TRPC）通道蛋白是一种非选择性阳离子通道蛋白家族，主要位于细胞膜表面，对钙离子具有通透性。研究认为，TRPC可能构成钙池操纵性钙通道（store-operated calcium channels, SOCC）并介导钙池操纵性钙内流（store-operated calcium entry, SOCE），从而参与细胞的增殖、迁移、基因转录等生命活动。本研究检测非小细胞肺癌（non-small cell lung cancer, NSCLC）组织中TRPC mRNA及蛋白质的表达情况，初步探讨TRPC与NSCLC的可能关系。
方法: 建立TRPC1-7等7个家族成员的荧光定量PCR检测方法，对24例NSCLC患者的肿瘤组织进行了TRPC mRNA的定量检测，并通过蛋白质免疫印迹法对TRPC在蛋白质水平的表达进行了验证。
结果: 在NSCLC患者癌组织检测到TRPC1、TRPC3、TRPC4和TRPC6 mRNA的表达，未检测到TRPC2、TRPC5和TRPC7 mRNA的表达。肺癌组织中TRPC表达丰度为：TRPC1≈TRPC6>TRPC3>TRPC4。蛋白质免疫印迹证实了非小细胞肺癌组织中TRPC1、TRPC3、TRPC4和TRPC6在蛋白质水平的表达。
结论: 非小细胞肺癌组织在mRNA和蛋白质水平均表达TRPC1、TRPC3、TRPC4和TRPC6，其中主要表达TRPC1和TRPC6，它们在构成肺癌细胞中SOCC、介导产生SOCE中的作用有待进一步研究。