Objective: To establish the HPLC fingerprints of Dipsacus asperoides for reflecting the internal information, evaluating its internal quality.
Methods: 20 batches of D. asperoides were collected from different place with the HPLC fingerprints method. Chromatographic column: Welchrom-C18 (250 mm x 4. 6 mm, 5 microm), mobile phase: acetonitrile and water (gradient elution), flow rate: 1.0 mL/min, detection wavelength: 212 nm, column temperature: 35 degrees C.
Results: The common mode of HPLC fingerprint was established and similar degrees to D. asperoides from different areas were compared.
Conclusion: The method is stable, reliable, and with full information which can be used for quality evaluation, quality control item and crude drug identification of D. asperoides.