Abstract
Neural stem cells have a broad differentiation repertoire during embryonic development and can be reprogrammed to pluripotency comparatively easily. We report that adult neural stem cells can be reprogrammed at very high efficiency to monocytes, a differentiated fate of an unrelated somatic lineage, by ectopic expression of the Ets transcription factor PU.1. The reprogrammed cells display a marker profile and functional characteristics of monocytes and integrate into tissues after transplantation. The failure to reprogram lineage-committed neural cells to monocytes with PU.1 suggests that neural stem cells are uniquely amenable to reprogramming.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium-Binding Proteins / metabolism
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Cell Line
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Cells, Cultured
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Cellular Reprogramming*
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Embryo, Mammalian / cytology
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Embryo, Mammalian / metabolism
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Female
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Immunohistochemistry
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Lentivirus / genetics
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Male
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Mice
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Mice, Inbred C57BL
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Microfilament Proteins
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Microscopy, Confocal
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Monocytes / cytology
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Monocytes / metabolism*
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Neurons / cytology
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Neurons / metabolism*
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Pregnancy
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Stem Cells / cytology
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Stem Cells / metabolism*
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Time Factors
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transduction, Genetic
Substances
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Aif1 protein, mouse
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Calcium-Binding Proteins
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Microfilament Proteins
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Proto-Oncogene Proteins
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Trans-Activators
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proto-oncogene protein Spi-1
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Green Fluorescent Proteins