Background and objective: It was proven that Vitamin C could inhibit the growth of many types of tumors as an antioxidant. The aim of this study is to explore role of Vitamin C in proliferation and apoptosis of lung carcinoma cell line A549 and the underlying mechanism.
Methods: A549 cells were cultured in vitro and incubated with Vitamin C. The cell viability was measured by growth curve and clonogentic assay. Flow cytometry was used to analyze cell cycle and detect apoptosis. The levels of expression of Caspase-3 mRNA and Survivin mRNA were detected by RT-PCR.
Results: Vitamin C of 400 microg/mL, 4 mg/mL significantly inhibited the growth of A549 cell lines (P = 0.024, P = 0.015, respectively). Flow cytometry showed that the cells major stagnation stayed in the G0/G1 and S phase and the apoptotic rate increased with time prolonged. Vitamin C signifiantly up-regulated the expression of Caspase-3 mRNA, but had no effect on Survivin mRNA.
Conclusion: Vitamin C can inhibit the proliferation of A549, block A549 cells in G0/G1 and S phase, and induce apoptosis of A549 cells. Apotosis occurred by up-regulated the expression of Caspase-3.
背景与目的: 维生素C作为一种抗氧化剂, 对多种肿瘤均有抑制作用, 本研究旨在探讨维生素C对肺癌细胞株A549的增殖、凋亡的影响及其诱导A549细胞凋亡的可能机制。
方法: 在体外培养的肺癌A549细胞株中加入不同浓度的维生素C, 采用细胞生长曲线及克隆形成实验检测细胞生长情况; 用流式细胞仪检测细胞周期的影响及凋亡率; 用RT-PCR方法检测肺癌细胞株A549中Caspase-3、Survivin的表达差异。
结果: 400 μg/mL、4 mg/mL浓度组维生素C明显抑制A549细胞的增殖, 流式细胞仪检测细胞被阻止在G0/G1期及S期, 且随着时间的延长细胞凋亡逐渐增多, RT-PCR检测维生素C可以上调Caspase-3 mRNA的表达, 并且随着时间的延长Caspase-3 mRNA的表达逐渐增强, 对Survivin mRNA的表达无确切作用。
结论: 维生素C呈时间和剂量依赖性抑制A549细胞的增殖, 并使A549细胞阻止在G0/G1期及S期, 并呈时间依赖性诱导A549细胞凋亡, 其机制可能是通过上调Caspase-3的表达。