Ralstonia pickettii isolated from copper-contaminated lake sediment are adapted to high levels of copper after 100 years of selective pressure. Two R. pickettii strains (12D and 12J) were selected for the studies reported herein due to their distinct differences in genomic structure, different metal resistance patterns and carriage of a filamentous phage. Copper sequestration studies revealed that these strains could bind up to 27.44 (12D) and 38.19 (12J) mg copper per g dry weight of cells and that viable cells sequestered more copper than heat-killed cells. Viable cells and heat-killed cells had significantly different saturation binding curves, indicating that one or more unique copper sequestration mechanism(s) was involved in binding by viable cells. Electron microscopy showed alteration of cell outer envelope after cells were grown in the presence of copper, suggesting that the accumulation of copper was membrane associated. X-ray Absorption Near Edge Structure and Extended X-ray Absorption Fine Structure revealed that the copper sequestered was present as Cu(II) and bound to oxygen and/or nitrogen. Recent completion of the genome sequence revealed that an approximately 220 kb region was enriched with metal resistance and transporter genes found in multiple copies. Comparative sequence analysis revealed that several genes may have been derived from horizontal transfer. Hence, rapid adaptation of R. pickettii to high concentrations of metal appears due to robust gene duplication and importation of several types of resistance determinants.