Proteome profile of the developing maize (Zea mays L.) rachis

Proteomics. 2010 Aug;10(16):3051-5. doi: 10.1002/pmic.200900833.

Abstract

In this study, we performed the first high-throughput proteomic analysis of developing rachis (cob) from maize genotype Mp313E. Using two proteomic approaches, 2-DE and 2-D LC, we identified 967 proteins. A 2-D proteome reference map was established. Functional classification of identified proteins revealed that proteins involved in various cellular metabolisms, response to stimulus and transport, were the most abundant.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Peptide Fragments / chemistry
  • Peptide Fragments / metabolism
  • Plant Proteins / chemistry*
  • Plant Proteins / metabolism
  • Proteome / chemistry*
  • Proteome / metabolism
  • Tandem Mass Spectrometry
  • Trypsin / metabolism
  • Zea mays / chemistry*

Substances

  • Peptide Fragments
  • Plant Proteins
  • Proteome
  • Trypsin