Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection

Biotechnol Prog. 2010 Nov-Dec;26(6):1528-33. doi: 10.1002/btpr.475.

Abstract

To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH(2)) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH(2) and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH(2) /DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amination
  • Antineoplastic Agents / chemical synthesis*
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Apoptosis / drug effects
  • Cell Survival / drug effects
  • DNA / chemistry*
  • Drug Carriers / chemical synthesis*
  • Drug Carriers / chemistry
  • Drug Carriers / pharmacology
  • Drug Screening Assays, Antitumor
  • Gene Transfer Techniques*
  • Green Fluorescent Proteins / chemistry
  • HeLa Cells
  • Humans
  • Materials Testing
  • Molecular Structure
  • Particle Size
  • Plasmids / chemistry
  • Polysorbates / chemical synthesis*
  • Polysorbates / chemistry
  • Polysorbates / pharmacology
  • Structure-Activity Relationship
  • Surface Properties
  • Transfection

Substances

  • Antineoplastic Agents
  • Drug Carriers
  • Polysorbates
  • Green Fluorescent Proteins
  • DNA