Abstract
A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus / enzymology*
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Base Sequence
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Bleaching Agents / metabolism*
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Chromatography, Gel
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Chromatography, Ion Exchange
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Chromatography, Liquid
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Chromatography, Thin Layer
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Cloning, Molecular
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Eucalyptus / chemistry
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Hydrogen-Ion Concentration
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Lignin / metabolism
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Molecular Sequence Data
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Oligosaccharides / metabolism*
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Sequence Analysis, DNA
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Temperature
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Xylosidases / genetics*
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Xylosidases / metabolism
Substances
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Bleaching Agents
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Oligosaccharides
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Kraft lignin
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Lignin
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Xylosidases