Potential anti-inflammatory diterpenoids from the roots of Caesalpinia mimosoides Lamk

Phytochemistry. 2010 Oct;71(14-15):1756-64. doi: 10.1016/j.phytochem.2010.06.016. Epub 2010 Jul 23.

Abstract

Anti-inflammatory assay-guided separation of extracts from the roots of Caesalpinia mimosoides Lamk. led to isolation of seven compounds: four diterpenes (1-4), a dimer (9), and two dibenzo[b,d]furans (10, 11) together with eleven known compounds. Their structures were elucidated by 1D- and 2D-NMR techniques as well as UV, IR, mass spectral data and comparison with literature values. The anti-inflammatory activities of all compounds were evaluated for inhibitory activities against lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 cell lines. Compounds 4, 6, 8, and 12-14 were also tested for the inhibitory effect on LPS-induced tumor necrosis factor-alpha (TNF-alpha) release in RAW264.7 cells. The results indicated that 4 possessed potent inhibitory activity for both tests with IC(50) values of 3.0 and 6.5 microM, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / chemistry
  • Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Caesalpinia / chemistry*
  • Diterpenes / chemistry
  • Diterpenes / isolation & purification*
  • Diterpenes / pharmacology*
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Mice
  • Molecular Structure
  • Nitric Oxide / biosynthesis
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Roots / chemistry
  • Thailand
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Diterpenes
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide