Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China

Diagn Microbiol Infect Dis. 2010 Aug;67(4):355-8. doi: 10.1016/j.diagmicrobio.2010.03.004.

Abstract

One hundred Mycoplasma pneumoniae strains were isolated from pediatric patients from March 2008 to July 2009. Of 100 isolates, 90 (90%) were resistant to erythromycin (MICs >128 microg/mL for 88 strains and 64 microg/mL for 2 strains), azithromycin, and clarithromycin. Fluoroquinolones and tetracyclines maintain good activities against clinical M. pneumoniae isolates. Of 90 macrolide-resistant M. pneumoniae strains, 88 (98%) harbored an A-to-G transition mutation at position 2063 in 23S rRNA genes, and the remaining 2 showed either A2064G or A2063T mutation; the latter point mutation is newly discovered and reported. Ninety-three (93%) clinical isolates were classified into the P1 gene restriction fragment length polymorphism (RFLP) type I, and 7 (7%) were type II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Azithromycin / pharmacology
  • Bacterial Typing Techniques
  • Child
  • Child, Preschool
  • China
  • Clarithromycin / pharmacology
  • DNA Fingerprinting
  • Drug Resistance, Bacterial*
  • Erythromycin / pharmacology
  • Fluoroquinolones / pharmacology
  • Genotype
  • Humans
  • Macrolides / pharmacology*
  • Microbial Sensitivity Tests
  • Mycoplasma pneumoniae / classification
  • Mycoplasma pneumoniae / drug effects*
  • Mycoplasma pneumoniae / genetics
  • Mycoplasma pneumoniae / isolation & purification
  • Pneumonia, Mycoplasma / microbiology*
  • Point Mutation
  • Polymorphism, Restriction Fragment Length
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 23S / genetics
  • Tetracyclines / pharmacology

Substances

  • Anti-Bacterial Agents
  • Fluoroquinolones
  • Macrolides
  • RNA, Bacterial
  • RNA, Ribosomal, 23S
  • Tetracyclines
  • Erythromycin
  • Azithromycin
  • Clarithromycin