Abstract
The ability of Pseudomonas fluorescens 26K strain to utilize naphthalene at concentrations up to 600 mg/liter as the sole source of carbon and energy in mineral liquid media was shown. Using HPLC, TLC, and mass-spectrometry, the intermediates of naphthalene transformation by this strain were identified as naphthalene cis-1,2-dihydrodiol, salicylaldehyde, salicylate, catechol, 2-hydroxymuconic semialdehyde, and 1-naphthol. Catechol 2,3-dioxygenase (a homotetramer with native molecular mass 125 kDa) and NAD+-dependent homohexameric naphthalene cis-1,2-dihydrodiol dehydrogenase with native molecular mass 160 kDa were purified from crude extract of the strain and characterized. NAD+-dependent homodimeric salicylaldehyde dehydrogenase with molecular mass 110 kDa was purified and characterized for the first time. Based on the data, a pathway of naphthalene degradation by P. fluorescens 26K is suggested.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde Oxidoreductases / chemistry*
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Aldehyde Oxidoreductases / isolation & purification
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Aldehydes / analysis
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Catechol 2,3-Dioxygenase / chemistry*
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Catechol 2,3-Dioxygenase / isolation & purification
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Catechols / analysis
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Chromatography, High Pressure Liquid
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Electrophoresis, Polyacrylamide Gel
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Fatty Acids, Unsaturated / analysis
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Mass Spectrometry
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Naphthalenes / metabolism*
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Naphthols / analysis
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Oxidoreductases Acting on CH-CH Group Donors / chemistry*
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Oxidoreductases Acting on CH-CH Group Donors / isolation & purification
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Pseudomonas fluorescens / enzymology*
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Salicylates / analysis
Substances
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Aldehydes
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Catechols
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Fatty Acids, Unsaturated
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Naphthalenes
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Naphthols
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Salicylates
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salicylaldehyde
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1-naphthol
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hydroxymuconic semialdehyde
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1,2-dihydroxy-1,2-dihydronaphthalene
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Catechol 2,3-Dioxygenase
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Aldehyde Oxidoreductases
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salicylaldehyde dehydrogenase
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Oxidoreductases Acting on CH-CH Group Donors
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cis-1,2-dihydrobenzene-1,2-diol dehydrogenase
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catechol