Here we describe a new procedure for ascospore isolation from cultures containing a majority of unsporulated vegetative cells of Saccharomyces cerevisiae. The EZ ascospore isolation procedure relies on the combination of two conventional protocols, diethyl ether treatment and modified zymolyase treatment, allowing a significant increase in the efficiency of ascospore isolation and consequently enabling a large number of meiotic offspring to be efficiently obtained and screened, thus improving the efficacy of genetic research and the genetic selection of S. cerevisiae strains.
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