10-23 DNAzyme modified with (2'R)- and (2'S)-2'-deoxy-2'-C-methyluridine in the catalytic core

Bioorg Med Chem Lett. 2010 Aug 1;20(15):4367-70. doi: 10.1016/j.bmcl.2010.06.071. Epub 2010 Jun 16.

Abstract

The catalytic core of a 10-23 DNAzyme was modified using (2'R), (2'S)-2'-deoxy-2'-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg(2+) concentrations, indicating that certain 2'-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2'-C-methyl-2'-deoxynucleosides increased the stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Cell Line, Tumor
  • DNA, Catalytic / antagonists & inhibitors*
  • DNA, Catalytic / metabolism
  • DNA, Single-Stranded / antagonists & inhibitors*
  • DNA, Single-Stranded / metabolism
  • Humans
  • Magnesium / metabolism
  • Uridine / analogs & derivatives*
  • Uridine / chemical synthesis
  • Uridine / chemistry
  • Uridine / pharmacology

Substances

  • 2'-C-methyluridine
  • DNA, Catalytic
  • DNA, Single-Stranded
  • RNA-cleaving DNA 10-23
  • Magnesium
  • Uridine