Identification of newly described Streptococcus pneumoniae serotype 6D by use of the Quellung reaction and PCR

Shahin Oftadeh; Catherine Satzke; Gwendolyn L Gilbert

doi:10.1128/JCM.00675-10

Identification of newly described Streptococcus pneumoniae serotype 6D by use of the Quellung reaction and PCR

J Clin Microbiol. 2010 Sep;48(9):3378-9. doi: 10.1128/JCM.00675-10. Epub 2010 Jul 7.

Authors

Shahin Oftadeh¹, Catherine Satzke, Gwendolyn L Gilbert

Affiliation

¹ Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia. Shahin.oftadeh@swahs.health.nsw.gov.au

Abstract

We tested 121 pneumococcal serogroup 6 isolates (including 30 serotype 6C and 24 serotype 6D isolates) by serotype-specific PCR and the Quellung reaction, using "old" and "new" pool B, factor 6b, and new factor 6d antisera. In combination with group B and other factor antisera, factor 6d antiserum can reliably identify the newly described serotype 6D.

MeSH terms

Antibodies, Bacterial
Bacterial Typing Techniques / methods*
Humans
Pneumococcal Infections / microbiology*
Polymerase Chain Reaction / methods*
Serotyping
Streptococcus pneumoniae / classification*
Streptococcus pneumoniae / isolation & purification*

Substances

Antibodies, Bacterial