Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles

Gulsu Sener; Erdogan Ozgur; Erkut Yılmaz; Lokman Uzun; Ridvan Say; Adil Denizli

doi:10.1016/j.bios.2010.06.003

Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles

Biosens Bioelectron. 2010 Oct 15;26(2):815-21. doi: 10.1016/j.bios.2010.06.003. Epub 2010 Jun 11.

Authors

Gulsu Sener¹, Erdogan Ozgur, Erkut Yılmaz, Lokman Uzun, Ridvan Say, Adil Denizli

Affiliation

¹ Hacettepe University, Department of Chemistry, Ankara, Turkey.

PMID: 20605089
DOI: 10.1016/j.bios.2010.06.003

Abstract

The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37°C for 6h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 μg/mL) and natural sources (egg white) (460-1500 ng/mL).

MeSH terms

Biosensing Techniques / instrumentation*
Equipment Design
Equipment Failure Analysis
Micro-Electrical-Mechanical Systems / instrumentation*
Muramidase / analysis*
Nanoparticles / chemistry*
Nanoparticles / ultrastructure*
Nanotechnology / instrumentation*
Surface Properties

Substances

Muramidase