Direct and rapid electrochemical immunosensing system based on a conducting polymer

Talanta. 2010 Jul 15;82(2):608-12. doi: 10.1016/j.talanta.2010.05.015. Epub 2010 May 19.

Abstract

A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen-antibody interaction model using ovalbumin-anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen-antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented.

MeSH terms

  • Acetic Acid / chemistry
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Electrochemistry
  • Enzymes, Immobilized / chemistry
  • Naphthoquinones / chemistry
  • Polymers / chemistry*
  • Reference Standards
  • Serum / chemistry*
  • Sulfhydryl Compounds / chemistry
  • Time Factors

Substances

  • Enzymes, Immobilized
  • Naphthoquinones
  • Polymers
  • Sulfhydryl Compounds
  • Acetic Acid
  • 1,4-naphthoquinone