The present study investigated the effects of dietary linolenic acid (ALA) versus linoleic acid (LA) on meat quality, fatty acid composition, and stearoyl-CoA desaturase (SCD) activity in longissimus muscle (MLD) and subcutaneous adipose tissue (SAT) of German Holstein bulls and the transfer of beneficial n-3 fatty acids into German corned beef sausages (GCB). Feeding LA- and ALA-enriched diets increased essential fatty acids in MLD and SAT. The ALA-supplemented diet decreased significantly the SCD activity in MLD and SAT, resulting in a reduced relative concentration of oleic acid in muscle. The relative proportion of CLAcis-9,trans-11 analyzed by HPLC was not different between groups in either tissue. GCB were produced by using the lean meat of bulls. Beef products of bulls fed the ALA-supplemented diet were rich in ALA and n-3 LC PUFA. Most importantly, there was no loss of n-3 fatty acids during processing under production conditions. Conclusively, the n-6/n-3 fatty acid ratio was beneficially low.