Size-controlled cadmium sulfide nanoparticles were successfully synthesized by immobilized Rhodobacter sphaeroides in the study. The dynamic process that Cd(2+) was transported from solution into cell by living R. sphaeroides was characterized by transmission electron microscopy (TEM). Culture time, as an important physiological parameter for R. sphaeroides growth, could significantly control the size of cadmium sulfide nanoparticles. TEM demonstrated that the average sizes of spherical cadmium sulfide nanoparticles were 2.3 +/- 0.15, 6.8 +/- 0.22, and 36.8 +/- 0.25 nm at culture times of 36, 42, and 48 h, respectively. Also, the UV-vis and photoluminescence spectral analysis of cadmium sulfide nanoparticles were performed.