Abstract
Anti-HIV peptides with inhibitory activity against HIV-1 integrase (IN) have been found in overlapping peptide libraries derived from HIV-1 gene products. In a strand transfer assay using IN, inhibitory active peptides with certain sequential motifs related to Vpr- and Env-derived peptides were found. The addition of an octa-arginyl group to the inhibitory peptides caused a remarkable inhibition of the strand transfer and 3'-end-processing reactions catalyzed by IN and significant inhibition against HIV replication.
Publication types
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Line
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Cell Line, Tumor
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Genes, Viral
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HIV Integrase / chemistry
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HIV Integrase Inhibitors / chemistry*
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HIV Integrase Inhibitors / pharmacology
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HIV-1 / drug effects
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HIV-1 / genetics*
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HIV-1 / physiology
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Human Immunodeficiency Virus Proteins / chemistry*
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Humans
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Models, Molecular
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Peptide Library*
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Peptides / chemistry*
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Peptides / pharmacology
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Virus Replication / drug effects
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env Gene Products, Human Immunodeficiency Virus / chemistry*
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vpr Gene Products, Human Immunodeficiency Virus / chemistry*
Substances
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HIV Integrase Inhibitors
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Human Immunodeficiency Virus Proteins
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Peptide Library
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Peptides
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env Gene Products, Human Immunodeficiency Virus
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vpr Gene Products, Human Immunodeficiency Virus
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HIV Integrase
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p31 integrase protein, Human immunodeficiency virus 1