Analyzing binding data

Curr Protoc Neurosci. 2010 Jul:Chapter 7:Unit 7.5. doi: 10.1002/0471142301.ns0705s52.

Abstract

Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and accessibility of these binding sites for various drugs. This unit explains how to design and analyze such experiments.

MeSH terms

  • Binding, Competitive
  • Fluorescence
  • Fluorescence Polarization / methods
  • Fluorescence Resonance Energy Transfer / methods
  • Kinetics
  • Ligands
  • Linear Models
  • Models, Chemical
  • Nonlinear Dynamics
  • Protein Binding
  • Radioligand Assay / methods*
  • Radiopharmaceuticals / chemistry
  • Receptors, G-Protein-Coupled / agonists
  • Receptors, G-Protein-Coupled / chemistry
  • Regression Analysis
  • Spectrometry, Fluorescence / methods

Substances

  • Ligands
  • Radiopharmaceuticals
  • Receptors, G-Protein-Coupled