Objective: Application of Real Time Fluorescent quantitative reverse transcription polymerase chani reaction (rRT-PCR)to poliovirus identification in Chinese Poliomyelitis Laboratory Network and evaluation of the assay.
Methods: According to Real-time RT-PCR recommended by WHO and developed by USA Centers for Disease Control and Prevention, 10 poliovirus isolates from laboratories of Chinese poliomyelitis network were tested for intratypic differentiation (ITD) and vaccine derived polioviruses (VDPVs) screening. The results of Real-time RT-PCR for 10 isolates were compared with those of VP, region sequencing.
Result: The Real-time RT-PCR results for 10 isolates did not completely consist with those of VP1 region sequencing. 5 Pre-VDPVs can not be identified by Real-time RT-PCR and type IVDPV from Shanxi province in 2009 was missed by the assay.
Conclusion: The Real-time RT-PCR retrospective and prospective researches for large scale of polioviruses well be conducted to determine if the assay is applicable to Chinese Poliomyelitis Laboratory Network.