Biodegradation of chlorimuron-ethyl by the bacterium Klebsiella jilinsis 2N3

J Environ Sci Health B. 2010 Aug;45(6):501-7. doi: 10.1080/03601234.2010.493473.

Abstract

Enrichment culturing of sludge taken from an industrial wastewater treatment pond led to the identification of a bacterium (Klebsiella jilinsis H. Zhang) that degrades chlorimuron-ethyl with high efficiency. Klebsiella jilinsis strain 2N3 grows with chlorimuron-ethyl as the sole nitrogen source at the optimal temperature range of 30-35 degrees C and pH values between 6.0-7.0. In liquid medium, the degradation activity was further induced by chlorimuron-ethyl. Degradation rates followed the pesticide degradation kinetic equation at concentrations between 20 and 200 mg L(-1). Using initial concentrations of 20 and 100 mg L(-1), the degradation rates of chlorimuron-ethyl were 83.5 % and 92.5 % in 12 hours, respectively. At an initial concentration higher than 200 mg L(-1), the degradation rate decreased slightly as the concentration increased. The 2N3 strain also degraded the sulfonylurea herbicides ethametsulfuron, metsulfuron-methyl, nicosulfuron, rimsulfuron, and tribenuron-methyl. This study provides scientific evidence and support for the application of K. jilinsis in bioremediation to reduce environmental pollution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Kinetics
  • Klebsiella / classification
  • Klebsiella / genetics
  • Klebsiella / isolation & purification*
  • Klebsiella / metabolism*
  • Molecular Sequence Data
  • Pesticides / chemistry
  • Pesticides / metabolism*
  • Phylogeny
  • Pyrimidines / chemistry
  • Pyrimidines / metabolism*
  • RNA, Ribosomal, 16S / genetics
  • Sewage / microbiology*
  • Sulfonylurea Compounds / chemistry
  • Sulfonylurea Compounds / metabolism*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Pesticides
  • Pyrimidines
  • RNA, Ribosomal, 16S
  • Sewage
  • Sulfonylurea Compounds
  • chlorimuron ethyl