Abstract
The aim of this study was to evaluate the anti-tumor activity of Amblyomin-X, a serine protease Kunitz-type inhibitor. Amblyomin-X induced tumor mass regression and decreased number of metastatic events in a B16F10 murine melanoma model. Alterations on expression of several genes related to cell cycle were observed when two tumor cell lines were treated with Amblyomin-X. PSMB2, which encodes a proteasome subunit, was differentially expressed, in agreement to inhibition of proteasomal activity in both cell lines. In conclusion, our results indicate that Amblyomin-X selectively acts on tumor cells by inducing apoptotic cell death, possibly by targeting the ubiquitin-proteasome system.
Copyright © 2010 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / therapeutic use*
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Apoptosis / drug effects
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Arthropod Proteins
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Cell Cycle / drug effects
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Cell Line, Tumor
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Disease Models, Animal
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Gene Expression Regulation, Neoplastic / drug effects*
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Ixodidae / chemistry*
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Ixodidae / genetics
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Melanoma, Experimental / drug therapy*
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Melanoma, Experimental / pathology
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Mice
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Mice, Inbred C57BL
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Neoplasm Metastasis
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Proteasome Endopeptidase Complex / drug effects
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Proteasome Endopeptidase Complex / metabolism*
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Salivary Glands / chemistry
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Salivary Proteins and Peptides / chemistry
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Salivary Proteins and Peptides / isolation & purification
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Salivary Proteins and Peptides / therapeutic use*
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Serine Proteinase Inhibitors / chemistry
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Serine Proteinase Inhibitors / isolation & purification
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Serine Proteinase Inhibitors / therapeutic use*
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Ubiquitin / metabolism*
Substances
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Amblyomin-X protein, Amblyomma cajennense
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Antineoplastic Agents
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Arthropod Proteins
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Salivary Proteins and Peptides
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Serine Proteinase Inhibitors
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Ubiquitin
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Proteasome Endopeptidase Complex