Responses of abalone Haliotis diversicolor to sublethal exposure of waterborne and dietary silver and cadmium

Abstract

In this study, we examined the chronic waterborne and dietary exposure of silver (Ag) and cadmium (Cd) to the abalone Haliotis diversicolor using various endpoints such as growth and feeding rates, metal body burden, subcellular distribution, and metallothionein (MT) concentration over a period of 7 weeks of exposure. The growth and feeding rates of abalones were inhibited during the early stage of exposure to different extents, but then recovered to nearly the control levels. A large portion of Ag was redistributed to organelles and metal-rich granules from the cellular debris fraction, whereas cellular debris and metallothionein-like protein were the dominant pools for the storage of Cd, which remained comparable during the exposure period. The MT concentrations were significantly elevated (in a dose-dependent manner) within the first 2 weeks of exposure, after which the MT concentrations started to decrease. All these results implied that abalones respond rapidly to metal exposure, but apparently developed subsequent acclimation.