Umbilical cord blood CD45(+)CD34(+) cells coexpression in preterm and full-term neonates: a pilot study

J Matern Fetal Neonatal Med. 2011 Feb;24(2):229-33. doi: 10.3109/14767058.2010.482606. Epub 2010 Jun 23.

Abstract

Objective: Human umbilical cord blood (hUCB) is rich in stem cells. The CD45(+)/CD34(+) coexpression in hUCB is a marker of hemopoietic progenitor cells. The objective of this study is to compare the coexpression of hUCB CD45(+)/CD34(+) cells in preterm (PT) and full-term (FT) neonates.

Methods: We studied the coexpression of hUCB CD45(+)/CD34(+) cells in PT and FT neonates. The study included 25 PT (29-36 weeks gestation) and 25 FT (37-41) neonates delivered at Ain Shams University, Maternity Hospital. Absolute mononuclear layer cord blood CD45(+)/CD34(+) cell count were measured by flow cytometry. Morbidity was assessed for 12 of the studied 25 PT infants, using Morbidity Assessment Index for Newborns score.

Results: The absolute CD45(+)/CD34(+) count did not differ between PT and FT infants: Z = -0.485, p = 0.63. There was no correlation between absolute cell count and GA (r = 0.013, p = 0.9) for all 50 neonates. Mode of delivery did not affect the absolute count in the PT infants: Z = -0.6, p = 0.57. There was no correlation between the degree of morbidity and absolute cell count in PT neonates; r = 0.13, p = 0.69.

Conclusion: The absolute cell count is not affected by gestational age and did not relate to morbidity scores in the studied PT infants. Further, wide-scale work will be needed to study CD45(+)/CD34(+) count in hUCB in sick PT neonates.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, CD34 / metabolism*
  • Blood Cell Count
  • Blood Cells / cytology
  • Blood Cells / metabolism
  • Fetal Blood / cytology*
  • Fetal Blood / metabolism*
  • Flow Cytometry
  • Gestational Age
  • Humans
  • Infant, Newborn / blood
  • Infant, Newborn / metabolism
  • Infant, Premature / blood
  • Infant, Premature / metabolism
  • Leukocyte Common Antigens / metabolism*
  • Pilot Projects
  • Premature Birth* / blood
  • Premature Birth* / metabolism
  • Term Birth* / blood
  • Term Birth* / metabolism

Substances

  • Antigens, CD34
  • Leukocyte Common Antigens
  • PTPRC protein, human