Abstract
Dendritic cells (DCs) are very important for the generation of long lasting immune responses against pathogens or the induction of anti-tumor responses. Targeting antigen to dendritic cells via monoclonal antibodies specific for DC cell surface receptors such as DEC205 was shown to elicit potent cellular and humoral immune responses in vivo. Therefore, we investigated whether this novel strategy might also be useful for the generation of new monoclonal antibodies against molecules of choice. We show, that by targeting the extracellular domain of the human C-type lectin receptor ClecSF6/DCIR/LLIR (hDCIR) to DEC205 on DCs in vivo, we were able to generate highly specific monoclonal antibodies against hDCIR.
Copyright 2010 Elsevier B.V. All rights reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / immunology
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Antigen Presentation / immunology
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CD11c Antigen / metabolism
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Cell Differentiation / immunology
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Female
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Humans
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Lectins, C-Type / chemistry
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Lectins, C-Type / immunology*
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Lectins, C-Type / metabolism
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / immunology*
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred C57BL
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Receptors, Immunologic / chemistry
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Receptors, Immunologic / immunology*
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Receptors, Immunologic / metabolism
Substances
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Antibodies, Monoclonal
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CD11c Antigen
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CLEC4A protein, human
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Lectins, C-Type
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Membrane Glycoproteins
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Receptors, Immunologic