Aim: To explore the immunogenicity of mouse neural stem cells (NSCs), thus help to solve the problem of immunologic rejection in NSCs transplantation.
Methods: (1) Thirty C57BL/6 mice were assigned randomly into two groups, then the two groups were immuned by intraperitoneal injection respectively with BALB/c mice's NSCs or hepatic cells (as control). Afterwards one-way mixed lymphocyte culture was performed with BALB/c mice's NSCs or hepatic cells and immuned C57BL/6 mice's T lymphocytes. Immune response of T lymphocyte was detected by liquid scintillation counter. (2) The MHC-I and MHC-II molecules of BALB/c mice NSCs were stained with FITC- and PE-labeled antibody respectively. Then the expression of MHC-I and MHC-II were detected by flow cytometry.
Results: As shown by liquid scintillation counter, the cpm value of NSCs was 16592.8 +/- 2865.3, and that of the hepatic cells was 27815.0 +/- 2416.3 (P<0.01). About (87.55 +/- 3.65)% NSCs expressed neither MHC-I nor MHC-II, but there were only about (27.45 +/- 1.86)% hepatocytes showed both MHC-I and MYC-II negative (P<0.01).
Conclusion: The mouse NSCs show weaker immunogenicity and their immunogenicity is significantly weaker than their hepatocytes.