Abstract
We investigated the effects of treatment with trimethyltin (TMT) on the expression of glutathione-related enzymes in mouse hippocampus. TMT promoted the expression of glutathione S-transferase (GST) Ya/Yc mRNA, and GSTA2 protein, but not that of glutamate-cysteine ligase catalytic subunit mRNA, 1 day after injection. TMT produced a slight but significant elevation of GST activity during the period from day 1 to 7 post-treatment. No significant change was seen in the activity of glutathione peroxidase at anytime post-TMT treatment. Our data suggest the prolonged elevation of GST activity in the hippocampus following TMT treatment through enhanced expression of the GST Ya/Yc.
MeSH terms
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Animals
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Gene Expression Regulation, Enzymologic / drug effects*
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Glutamate-Cysteine Ligase / genetics
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Glutamate-Cysteine Ligase / metabolism
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Glutathione / metabolism*
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Glutathione Peroxidase / metabolism
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Glutathione Transferase / genetics
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Glutathione Transferase / metabolism*
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Hippocampus / drug effects*
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Hippocampus / enzymology*
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Hippocampus / metabolism
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History, 16th Century
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Isoenzymes / genetics
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Isoenzymes / metabolism
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Male
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Mice
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Mice, Inbred Strains
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Oxidants / toxicity*
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Oxidative Stress / drug effects
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Time Factors
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Trimethyltin Compounds / toxicity*
Substances
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Isoenzymes
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Oxidants
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RNA, Messenger
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Trimethyltin Compounds
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trimethyltin
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Glutathione Peroxidase
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glutathione S-transferase Yc
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Glutathione Transferase
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glutathione S-transferase alpha
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Glutamate-Cysteine Ligase
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Glutathione