Abstract
Bovine mastitis caused by Mycoplasma bovis is of great economic importance to the beef and dairy industry. Here we describe a new specific real-time PCR assay targeting the uvrC gene that was developed to directly detect M. bovis from milk and tissue samples without laborious DNA purification.
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Cattle
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DNA Primers / genetics
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Endodeoxyribonucleases / genetics
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Female
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Lung / microbiology
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Lymph Nodes / microbiology
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Mammary Glands, Animal / microbiology
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Mastitis, Bovine / diagnosis
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Mastitis, Bovine / microbiology
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Milk / microbiology*
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Mycoplasma Infections / diagnosis
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Mycoplasma Infections / microbiology*
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Mycoplasma Infections / veterinary
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Mycoplasma bovis / genetics*
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Mycoplasma bovis / isolation & purification
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Polymerase Chain Reaction / methods*
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Bacterial Proteins
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DNA Primers
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Endodeoxyribonucleases