Direct detection of Mycoplasma bovis in milk and tissue samples by real-time PCR

Mol Cell Probes. 2010 Oct;24(5):321-3. doi: 10.1016/j.mcp.2010.05.001. Epub 2010 Jun 2.

Abstract

Bovine mastitis caused by Mycoplasma bovis is of great economic importance to the beef and dairy industry. Here we describe a new specific real-time PCR assay targeting the uvrC gene that was developed to directly detect M. bovis from milk and tissue samples without laborious DNA purification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Cattle
  • DNA Primers / genetics
  • Endodeoxyribonucleases / genetics
  • Female
  • Lung / microbiology
  • Lymph Nodes / microbiology
  • Mammary Glands, Animal / microbiology
  • Mastitis, Bovine / diagnosis
  • Mastitis, Bovine / microbiology
  • Milk / microbiology*
  • Mycoplasma Infections / diagnosis
  • Mycoplasma Infections / microbiology*
  • Mycoplasma Infections / veterinary
  • Mycoplasma bovis / genetics*
  • Mycoplasma bovis / isolation & purification
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Bacterial Proteins
  • DNA Primers
  • Endodeoxyribonucleases