The seroprevalence of toxoplasmosis in pigs was examined in China's southern Guangdong Province from March 2008 through May 2009. A total of 1,022 serum samples was collected from 12 administrative cities and assayed for Toxoplasma gondii antibodies by enzyme-linked immunosorbent assay test. The seroprevalence ranged from 0 to 58.1% and the average T. gondii antibody-positive prevalence was 27.0% (276/1,022), with breeding boars having the highest level at 39.6% (38/96) followed by breeding sows at 36.9% (52/141). The results of the present survey indicate that T. gondii infection is a significant health problem in pigs and represents a public health concern in southern China.